Composite

Part:BBa_M36085:Design

Designed by: Ashley Hammerbacher Celina Malavé   Group: Stanford BIOE44 - S11   (2014-04-30)


Winter Flounder Antifreeze Protein Actuator With Secretion Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
    Illegal BamHI site found at 102
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 123
    Illegal NgoMIV site found at 141
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to make sure that we had everything that could express the protein in yeast (Kozac sequence, Yeast secretion Tag) and optimized our sequences for yeast.


Source

The AFP comes from Uniprot and the parts come from the parts registry.

References